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KMID : 0380020040190060484
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 6 p.484 ~ p.488
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Production of Organic Acids from Food By-Products -Mass Production of Organic Acids by Continuous Flow Cell Recycling Fermentation-
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Chu Yun-Sang
Jin Sun-Ja
Hwang Pil-Ki
Choi Cheol-Ho
Lee Eui-Sang
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Abstract
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Fermentation studies were conducted in batch and continuous flow cell-recycle reactors with food by-products as substrates. The genus Propionibacterium acidipropionici ATCC 4965 was utilized in the production of organic acids. Good performance was achieved in the batch fermentation using hydrol as a carbon source and corn steep liquor (CSL) as nitrogen and vitamin sources. Product yields and productivity based on maximum values were 0.80 g total acids/g glucose and 0.26 g total acids/L/h, respectively, when , (w/v) of hydrol and , (w/v) of CSL were utilized. Continuous fermentation with cell-recycling system using the optimum amounts of substrates resulted in dramatic increase in cell concentration (X) and maximum productivity (P). Compared to the batch fermentation, X and P were increased by as much as 21 and 13 times, respectively, at the dilution ratio of , indicating that cell recycling fermentation of food by-products provides valuable means for the mass production of organic acids as well as utilizing cell mass as good nutrient resources.
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KEYWORD
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Propionibacterium acidipropionici, food by-products, organic acids, CSL, corn steep Liquor, hydrol, cell-recycling fermentation
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